Journal: Cell Reports Medicine

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state

doi: 10.1016/j.xcrm.2024.101869

Figure Lengend Snippet: Miltefosine enhances CAR-T cell efficacy against solid tumors in vivo (A) Experimental timeline of CDX model and the fold change of tumor volume in CAR-T cell-treated CDX mouse model in the presence or absence of miltefosine administration (PBS group, n = 3; other groups, n = 5). (B) Tumor weight of each group at the end of experiment (PBS group, n = 3; other groups, n = 5). (C) The percentages of mesothelin- and hCD3-positive area in tumor tissues (PBS group, n = 2; other groups, n = 5). Scale bar: 200 μm. (D) The fold change of tumor volume in CDX mouse model treated with CAR-T cells derived from another donor, in the presence or absence of miltefosine or anti-PD-1 antibody treatment ( n = 4). (E) The proportion of hCD3 + T cells in the peripheral blood of CDX mouse model ( n = 4). (F) The fold change of tumor volume in CAR-T cell-treated PDX mouse model, in the presence or absence of miltefosine or anti-PD-1 antibody treatment ( n = 4). (G) The proportion of hCD3 + T cells in the peripheral blood of PDX mouse model ( n = 4). Unpaired t test was used in statistical analysis. (H) Experimental timeline of the OT-1-treated melanoma isograft tumor model. (I) The fold change in tumor volume in the OT-1-treated melanoma isograft tumor model in the presence or absence of miltefosine administration ( n = 7). Unpaired t test was used in statistical analysis. NS, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All error bars denote SEM. See also Figure S7 .

Article Snippet: Rabbit anti- human CD3 Antibody (clone EP41) , ZSGB-BIO , Cat#ZA-0503.

Techniques: In Vivo, Derivative Assay

Journal: Cell Reports Medicine

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state

doi: 10.1016/j.xcrm.2024.101869

Figure Lengend Snippet:

Article Snippet: Rabbit anti- human CD3 Antibody (clone EP41) , ZSGB-BIO , Cat#ZA-0503.

Techniques: Functional Assay, Flow Cytometry, Control, Recombinant, Lysis, Activation Assay, Transfection, Luciferase, Viability Assay, SYBR Green Assay, RNA Sequencing Assay, Construct, Plasmid Preparation, Software